ABSTRACT
AIM: To explore the correlation by analyzing and comparing the expression of inflammatory factors in peripheral blood of human leukocyte antigen-B27(HLA-B27)positive and negative uveitis patients.METHODS: All 76 patients first diagnosed with uveitis in our hospital from January 2020 to April 2022 were screened in this retrospective study. Nucleated cells were isolated from human venous blood, and HLA-B27 was detected by flow cytometry(direct immunofluorescence), the patients were divided into the HLA-B27-positive group(≥90%)in 35 cases and HLA-B27-negative group(≤5%)in 41 cases. The whole blood RNA was extracted. The mRNA expression of interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-10(IL-10), protease activated receptor 2(PAR2), tumor necrosis factor-α(TNF-α), interleukin enhancer-binding factor -3(ILF3)were detected and compared by RT-qPCR.RESULTS: The IL-1β, IL-10, PAR2 and TNF-α mRNA were observed no difference between the two groups of patients(all P>0.05). The IL-6 mRNA in the patients of HLA-B27-positive group was higher than in the HLA-B27-negative group, the ILF3 mRNA was lower than that in the negative group(all P<0.05).CONCLUSION: The expression level of IL-6 in peripheral blood was significantly increased and ILF3 was decreased in HLA-B27-positive group, which can be used as auxiliary indicators for diagnosis and treatment of HLA-B27-positive uveitis.
ABSTRACT
OBJECTIVE@#To explore the effect of Chinese massage on the rehabilitation of scoliosis patients undergoing 3D printing orthopedics.@*METHODS@#A retrospective selection of 262 patients with adolescent idiopathic scoliosis(AIS) who underwent 3D printing technology orthopedics admitted to the Department of Orthopedics in our hospital from January 2013 to January 2019 were selected for clinical research. According to the rehabilitation treatment methods adopted by the patients after the operation, the patients were divided into control group and observation group, there were 131 cases in each group. The observation group was treated with traditional Chinese acupuncture and massage after operation, and the control group was treated with conventional rehabilitation. The torso rotation angle and the maximum Cobb angle before and after intervention were measured and compared between two groups, Oswestry Disability Index(ODI) was used for functional evaluation, and the visual analogue scale(VAS) was used to evaluate the changes in pain before and after rehabilitation intervention.@*RESULTS@#After the intervention, the trunk rotation angle and maximum Cobb angle of the observation group were significantly better than those of the control group, the VAS score of the observation group was significantly lower than that of the control group, ODI in the observation group was significantly lower than that in the control group, and the difference was statistically significant(P<0.05).@*CONCLUSION@#After scoliosis patients undergo preoperative 3D printing correction, the scientific and reasonable implementation of TCM acupuncture and massage can effectively improve the patient's vertebral rotation angle, maximum Cobb angle, and improve the patient's spinal function.
Subject(s)
Adolescent , Humans , Lumbar Vertebrae/surgery , Massage , Medicine, Chinese Traditional , Orthopedic Procedures , Orthopedics , Printing, Three-Dimensional , Retrospective Studies , Scoliosis/surgery , Spinal Fusion , Treatment OutcomeABSTRACT
This study is to provide the basis of establishing a quality evaluation system, based on the differences in appearance and internal components of Astragali Radix from different sources. The diameter of 18 batches of Astragali Radix, the content of alcohol(water) extract and 7 kinds of flavonoids were determined. The peak area ratio of flavonoid aglycon to aglycone was calculated. PCA and CA were carried out by synthesizing various indexes. The results of PCA and CA showed that Astragali Radix was obviously clustered into three types. Alcohol extract, formononetin/formosan glycosides,(pilose isoflavones+astragalus flavonoid A)/pilose isoflavone glucoside are the most significant differences in the variable importance projection index(VIP) of Astragali Radix. Combining the diameter, alcohol(water) extract, flavonoid aglycon to aglycone peak area ratio can provide an analysis method for the establishment of the grade evaluation system of Astragali Radix.
Subject(s)
Astragalus Plant , Drugs, Chinese Herbal , Glucosides , Glycosides , Plant RootsABSTRACT
Objective To observe the deposition of PM2.5 in the contact lens and its influence on the oxygen permeability and refractive index of the contact lens.Methods Atmospheric PM2.5 was collected for preparation of 1 mg · mL-1 PM2.5 suspension.Then,18 rigid gas permeable contact lenses (RGPCL) and 18 soft contact lens lenses (SCL) were grouped in control,experimental and PBS-rinse group,with 6 RGPCL and 6 SCL in each group.RGPCL and SCL in the experimental and PBS-rinse groups were incubated in 1 mg · mL-1 PM2.5 solution for 24 h,followed by PBS-rinse group were further washed for 1 h in PBS,whereas the controls were incubated in PBS for 24 h.All of them were examined with field scanning electron microscopy.Meanwhile,oxygen permeability and refractive index of contact lens were measured using the polar graphic devices and refractometry respectively.All the obtained data were quantitatively and statistically analyzed.Results After treatment in PM2.5 solution,there was a large amount of PM2.5 deposition on the RGPCL surface in the experimental group,and the amount of PM2.5 was counted (3.19 ± 1.64) · 100 μm-2,while no atmospheric particulate matter was found in the control group;following the treatment of PBS,a lot of PM2.5 attached on the surface of RGPCL remained visible,and the number of particles was (5.12 ± 1.27) · 100 μm-2.And the difference was statistically significant between the control group and PBS-rinse group (P ＜0.05).In addition,and the amount of PM2.5 attached on the surface of SCL in the exPerimental group was (2.16 ± 1.19) · 100 μm-2,while there wasn t any atmospheric particulate matter in the control group;and,after a wash of SCL in PBS,the amount of PM2.5 on SCL surface was (0.56 ±0.39) · 100 μm-2 in the PBS-rinse group,indicating a statistically difference between the control group and PBS-rinse group (P ＜0.05).Moreover,the amount of PM2.5 attachment on SCL surface in the experimental group was significantly lower than that of the control group,and the difference was statistically significant (P ＜ 0.05).After PM2.5 solution treatment,oxygen permeability of RGPCL and SCL was 100.00 ± 3.17 and 42.00 ± 2.57 respectively in the experimental group,as well as 100.00 ±2.36 and 41.00 ± 3.44 in the control group,with no statistically significant difference between the two groups (all P ＞ 0.05);while refractive index of RGPCL and SCL was 1.415 6 ±0.000 4 and 1.3737 ±0.000 7 respectively in the experimental group,as well as 1.415 3 ±0.000 4 and 1.373 7 ±0.000 l in the control group,with no statistically significant difference between the two groups either (all P ＞ 0.05).Conclusion PM2.5 can be attached to the surface of RGPCL and SCL,and PM2.5 is easier to adhere to RGPCL surface and is not easy to elute when compared with SCL,but PM2.5 deposition do not affect oxygen permeability and refractive index of contact lenses over a short span of time.
ABSTRACT
Abstract?AIM: To investigate the influence of propylene glycol mannite sulfate ( PGMS ) on the expression of tumor necrosis factor -α( TNF-α) and interleukin-1β( IL-1β) , in diabetic retinopathy by a rat model, to study the mechanism of PGMS against diabetic retinopathy, and provide a reliable theoretical and experimental evidence for the PGMS to be applied to clinical prevention and treatment of diabetic retinopathy.?METHODS: Male Wistar rats were randomized into 4 groups, normal control group, diabetic control group and PGMS in group, the PGMS in groups included the doses of 50mg/kg and 100mg/kg. 1% streptozotocin ( STZ) of 60 mg/kg was intraperitoneally injected in rats to establish the diabetic models. The PGMS with the doses of 50mg/kg and 100mg/kg were used to gavage in different groups of models for 12wk.Twelve weeks later, the animals were sacrificed and retinas were isolated. The aqueous humors and serums were taken, expressions of TNF-αand IL-1βprotein in retinas, aqueous humors and serums were detected by enzyme-linked immunosorbent assay ( ELISA) , respectively.The location and the expression of TNF-αand IL-1βprotein in retina tissue was detected by immunohistochemistry.?RESULTS: Twelve weeks after the use of PGMS, the level of blood glucose was not changed.ELISA showed that the expression of TNF-αand IL-1βprotein in serum and retina was significantly increased in diabetic control group than in normal control group(P<0.05), but in the groups which PGMS was given reduced, lower than those in diabetes mellitus( DM) group, especially as the concentration of PGMS increased ( P<0.05 ).But the levels of aqueous humor's TNF-αand IL-1βproteins in PGMS group were not reduced.Immunohistochemistry showed that the TNF -α protein was almost not expressed in normal control group. But the TNF-αprotein was highly expressed in diabetic control group. The expression mainly located in the ganglion cell layer, the inner plexiform layer, outer plexiform layer and pigment epithelium. The TNF-αprotein was weakly expressed at the group of 50mg/kg PGMS, the TNF-αprotein was almost not expressed at the group of 100mg/kg PGMS.When the normal control group was detected, the IL-1βprotein was weakly expressed in the outer plexiform layer.But the IL-1βprotein was also highly expressed in diabetic control group.The expression mainly located in the inner plexiform layer, outer plexiform layer and pigment epithelium. The IL -1βprotein was weakly expressed at the group of 50mg/kg and 100mg/kg PGMS.?CONCLUSION:PGMS can treat the diabetic retinopathy by downregulating the expressions of TNF-αand IL-1βin early diabetic retinopathy.PGMS maybe have a good control effect on early diabetic retinopathy.
ABSTRACT
Objective To understand the level and distribution of antibody F1 against plague in population of Ningxia natural plague foci in 2007 and 2008. Methods Seven hundred and eighteen blood samples were collected in five major cities and counties of natural plague foci, and 475 blood samples were collected in nonplague area as control group. Conventional indirect hemagglutination, colloidal gold test, and enzyme-linked immunoassay were employed to test the antibody. If the result was tested positive by more than two methods used then the result was defined as positive. Antibody titer that did not reach the positive standard was defined as suspected samples. Results A total of 718 serum samples were tested, the results showed that 9 samples were positive (antibody titer was 1:16 - 1:64), the positive rate was 1.25%(9/718), suspected samples was 28, the detection rate was 3.90%(28/718). Four hundred and seventy-five serum samples in the non-plague area were all negative by the three methods. There was a significant difference of antibody F1 positive rate between residents in historical epidemic area and history nonepidemic area(χ2 = 4.44, P< 0.05). There was no statistical significance of the positive rate[1.25%(9/718), 1.25%(9/718),2.51%(18/718)]among the three methods used(χ2 = 1.91, P> 0.05). Conclusion There still exists a certain proportion of Fl antibody positive people in Ningxia natural plague foci, and these people are distributed in areas where several animal plague prevalent in recent years.